AP Conjugate, Anti-(Mouse IgG+IgM), Raised in Goat from Applied Biosystems
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| Cat No. | Size | Price |
|---|---|---|
| T2192 | N/A mL | ¥12,000 |
データ読み込み : 2011-08-26
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- Category : Products > Protein Research > ELISA & Western Blotting > Substrates & Reagents > Alkaline Phosphatase Conjugates
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Alkaline phosphatase conjugated secondary antibody for use with the ELISA-Light™ Immunoassay and Western-Light™ or Western-Star™ Western Blotting Detection SystemsApplied Biosystems has optimized its alkaline phosphatase secondary antibody conjugates for western blotting or immunoassay procedures incorporating chemiluminescent 1,2-dioxetane enzyme substrates. These labeled secondary antibodies ensure minimal nonspecific cross-reactivity.
- Optimized for use in blotting applications with dioxetane-based substrate solutions (CSPD® or CDP-Star® substrates), providing consistent experiment-to-experiment results
- High specific activity of conjugates provides high sensitivity of detection with dioxetane-based substrate solutions (CSPD® or CDP-Star® substrates)
- Low non-specific binding provides highest possible signal-to-noise in western blotting and immunoassay applications
The recommended initial working dilution for the labeled antibodies is 1:5,000 for AP conjugates and 1:10,000 for biotinylated AP conjugates. Avidx-AP™ conjugate is a streptavidin-alkaline phosphatase conjugate developed for detection of biotinylated molecules. The conjugate is tested and optimized for low background with chemiluminescent detection procedures. Avidx-AP™ conjugate is also sold in conjunction with biotinylated molecular weight markers. The suggested working dilution of Avidx-AP™ conjugate is from 1:5,000 to 1:20,000.
